Pictures: Fungi Get Into the Holiday Spirit


Photograph courtesy Stephanie Mounaud, J. Craig Venter Institute

Mounaud combined different fungi to create a Santa hat and spell out a holiday message.

Different fungal grow at different rates, so Mounaud's artwork rarely lasts for long. There's only a short window of time when they actually look like what they're suppose to.

"You do have to keep that in perspective when you're making these creations," she said.

For example, the A. flavus fungi that she used to write this message from Santa grows very quickly. "The next day, after looking at this plate, it didn't say 'Ho Ho Ho.' It said 'blah blah blah,'" Mounaud said.

The message also eventually turned green, which was the color she was initially after. "It was a really nice green, which is what I was hoping for. But yellow will do," she said.

The hat was particularly challenging. The fungus used to create it "was troubling because at different temperatures it grows differently. The pigment in this one forms at room temperature but this type of growth needed higher temperatures," Mounaud said.

Not all fungus will grow nicely together. For example, in the hat, "N. fischeri [the brim and ball] did not want to play nice with the P. marneffei [red part of hat] ... so they remained slightly separated."

Published December 21, 2012

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Obama, Congress Waving Bye-Bye Lower Taxes?













The first family arrived in the president's idyllic home state of Hawaii early today to celebrate the holidays, but President Obama, who along with Michelle will pay tribute Sunday to the late Sen. Daniel Inouye at the National Memorial Cemetery of the Pacific, could be returning home to Washington sooner than he expected.


That's because the President didn't get his Christmas wish: a deal with Congress on the looming fiscal cliff.


Members of Congress streamed out of the Capitol Friday night with no agreement to avert the fiscal cliff -- a massive package of mandatory tax increases and federal spending cuts triggered if no deal is worked out to cut the deficit. Congress is expected to be back in session by Thursday.


It's unclear when President Obama may return from Hawaii. His limited vacation time will not be without updates on continuing talks. Staff members for both sides are expected to exchange emails and phone calls over the next couple of days.


Meanwhile, Speaker of the House John Boehner is home in Ohio. He recorded the weekly GOP address before leaving Washington, stressing the president's role in the failure to reach an agreement on the cliff.


"What the president has offered so far simply won't do anything to solve our spending problem and begin to address our nation's crippling debt," he said in the recorded address, "The House has done its part to avert this entire fiscal cliff. ... The events of the past week make it clearer than ever that these measures reflect the will of the House."








Fiscal Cliff Negotiations Halted for Christmas Watch Video









Cliffhanger: Congress Heads Home after 'Plan B' Vote Pulled from House Floor Watch Video









Fiscal Cliff: Boehner Doesn't Have Votes for Plan B Watch Video





Senate Minority Leader Mitch McConnell echoed the sentiment while lamenting the failure to reach a compromise.


"I'm stuck here in Washington trying to prevent my fellow Kentuckians having to shell out more money to Uncle Sam next year," he said.


McConnell is also traveling to Hawaii to attend the Inouye service Sunday.


If the White House and Congress cannot reach a deficit-cutting budget agreement by year's end, by law the across-the-board tax hikes and spending cuts -- the so called fiscal cliff -- will go into effect. Many economists say that will likely send the economy into a new recession.


Reports today shed light on how negotiations fell apart behind closed doors. The Wall Street Journal, citing unnamed sources, reported that when Boehner expressed his opposition to tax rate increases, the president allegedly responded, "You are asking me to accept Mitt Romney's tax plan. Why would I do that?"


The icy exchange continued when, in reference to Boehner's offer to secure $800 billion in revenue by limiting deductions, the speaker reportedly implored the president, "What do I get?"


The president's alleged response: "You get nothing. I get that for free."


The account is perhaps the most thorough and hostile released about the series of unsuccessful talks Obama and Boehner have had in an effort to reach an agreement about the cliff.


Unable to agree to a "big deal" on taxes and entitlements, the president is now reportedly hoping to reach a "small deal" with Republicans to avoid the fiscal cliff.


Such a deal would extend unemployment benefits and set the tone for a bigger deal with Republicans down the line.


In his own weekly address, Obama called this smaller deal "an achievable goal ... that can get done in 10 days."


But though there is no definitive way to say one way or the other whether it really is an achievable goal, one thing is for certain: Republican leadership does not agree with the president on this question.


Of reaching an agreement on the fiscal cliff by the deadline, Boehner said, "How we get there, God only knows."



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Tax fight sends GOP into chaos



Now that very issue is tearing the GOP apart and making it an all-but-ungovernable majority for Speaker John A. Boehner (R-Ohio) to lead in the House.


Disarray is a word much overused in politics. But it barely begins to describe the current state of chaos and incoherence as Republicans come to terms with electoral defeat and try to regroup against a year-end deadline to avert a fiscal crisis.

The presidential election was fought in large measure over the question of whether some Americans should pay more in taxes. Republicans lost that argument with the voters, who polls show are strongly in favor of raising rates for the wealthy.

But a sizable contingent within the GOP doesn’t see it that way and is unwilling to declare defeat on a tenet that so defines them. Nor are they prepared to settle for getting the best deal they can, as a means of avoiding the tax hikes on virtually everyone else that would take effect if no deal is reached.

When Boehner tried to bend even a little, by proposing to raise rates on income over $1 million, his party humiliated him, forcing him Thursday night to abruptly cancel a vote on his “Plan B.”

“We had a number of our members who just really didn’t want to be perceived as having raised taxes,” Boehner said Friday. “That was the real issue.”

Whether and how the party can resolve the issue has implications going forward. It could determine Boehner’s viability — even his survival— as leader of the only part of the federal government controlled by the Republicans.

It also could set the terms of engagement for the battles that lie ahead, including such contentious ones as immigration and the fiscal 2013 spending bills, which are funded for only half the year and which expire March 31.

As things stand now, some worry that nothing short of a catastrophe could force a resolution.

“We have sunk to the lowest common denominator in order to get a deal — sheer panic,” said Republican strategist Ron Bonjean, a former aide to House and Senate leadership. “The reality of a stock market crash is probably the only way Washington will strike a deal. It is probably the only scenario that could likely force the speaker’s hand and allow for a deal driven by Democratic votes to pass the House.”

Which, of course, is not an ideal way to govern.

“The hard-core anti-tax conservatives in the GOP seem to believe that Barack Obama will be blamed if there is no agreement reached to avoid sequestration and the tax increases that are coming,” said Sheldon D. Pollack, a University of Delaware law and political science professor who has written a history of Republican anti-tax policy. “Calculated gamble? Or are they simply incapable of recognizing that they do not control the White House or the Senate, and hence do not have the ability to control the agenda? Sadly, I think it is the latter.”

Their intransigence alone is unlikely to sell the electorate on the Republican point of view on taxes.

“You have to make an argument. You have to go out there and engage. You can’t just simply assert a position,” said GOP pollster David Winston, who advises the House leadership. “Part of the dynamic for Boehner is that he’s trying to have the debate over economic policies that should have occurred during the election, and he also has to deal with this piece of legislation.”

As long as there has been a Republican Party, there has been at least a faction within it that has taken a hard-line stance on taxes, Pollack said. But it has not always had the upper hand.

Abraham Lincoln, the first Republican president, was also the first to put a national income tax into place, as a temporary means of funding the Civil War. Even then, House Ways and Means Chairman Thaddeus Stevens (now enjoying a return to popular consciousness as Tommy Lee Jones’s character in the movie “Lincoln”) denounced the idea of a graduated rate structure as a “strange way to punish men because they are rich.”

The 16th Amendment, which established the constitutionality of the federal income tax in 1913, was proposed by a Republican, Senate Finance Committee Chairman Nelson W. Aldrich. But it was decried by another one, Sen. Henry Cabot Lodge of Massachusetts, as “confiscation of property under the guise of taxation” and “a pillage of a class.”

The divisions went on until the 1980 election of Ronald Reagan, who ran as an advocate of tax-cutting supply-side economics.

In 1990, Newt Gingrich established himself as the de facto head of his party in the House, when he stood up to a president of his own party and led the opposition to George H.W. Bush’s tax increases.

Gingrich insisted in an interview Friday that the Republicans still have leverage, if they are willing to fight hard enough.

“They need a strategy, not just a way of getting through this week,” he said.

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Football: Barca battle on without Vilanova for time being






MADRID: Barcelona will have to continue their march at the top of La Liga without Tito Vilanova after it was revealed the coach has suffered a relapse of a tumour on which he was operated just over a year ago.

The 44-year-old who took over from Pep Guardiola at the beginning of the season was thought to have fully recovered from surgery last November to remove the growth on his parotid gland, and he was back at work within 15 days.

However after an operation on Thursday it is thought he will have treatment for at least six weeks and he may or may not be available to return to work at some stage during that time.

Until then, Sporting director Andoni Zubizarreta confirmed on Thursday, the side will be led by Vilanova's assistant Jordi Roura.

"Without going into too much detail about the sporting side today and whether we win or not at Valladolid on Saturday, just to say we'll continue with our coaching staff and our way of working, we'll be in extraordinary hands and Tito I'm sure will be watching us on TV," he said.

Barca president Sandro Rossell added "the most important thing for the club at this moment is the full recovery of Tito".

Vilanova has led Barcelona to the most successful start to a season in La Liga's history, with 15 wins and one draw in 16 matches so far.

With the final round of La Liga games of 2012 brought forward to allow the players an extra day of Christmas holidays, Barca will make it a great finish to the year, on the pitch at least, if they take three points at Valladolid.

Last weekend's win over closest rivals Atletico Madrid increased the Catalan side's lead to nine points and with Real Madrid's surprise draw with Espanyol leaving them 13 points back in third, for some La Liga title is already destined for the Nou Camp in May.

That win was followed with the news on Monday that Carles Puyol and Xavi Hernandez will stay at Barca until at least 2016 and Lionel Messi until 2018, and it was all looking positive until Wednesday's shock news.

Last Sunday's clear dominance over their closest rivals Atletico Madrid, especially in the second-half of the 4-1 win, left most observers concluding that it is the Catalan side's title to lose.

Meanwhile, Real Madrid face a tricky trip to fourth placed Malaga less than a week after manager Jose Mourinho said the title was "almost impossible now".

Defeats at Getafe, Sevilla and Betis, followed by the draw with Espanyol last Sunday, have left Madrid far adrift from table-toppers Barca.

One player the 'Merengues' missed last Sunday was French forward Karim Benzema.

Benzema is closer to a return than colleague Gonzalo Higuain, but could still miss the trip to Malaga with a foot injury. However he has pledged his future to the club this week, despite his name being linked with Paris Saint-Germain.

Malaga come into the game with the best defensive record in the league having conceded only 10 goals in 16 games and full-back Nacho Monreal is confident.

"We're going to be up for the Madrid match, we're doing well, we're at home and we know that Madrid is not going too good, but they're a good team and it will be difficult. I just hope we can take our chances and get the three points," he said.

In this week's matches already played, Rayo Vallecano beat Levante 3-0, Real Sociedad edged Sevilla 2-1 and Espanyol defeated Deportivo La Coruna 2-0, on Thursday.

On Friday, substitute Adrian Lopez hit an important winner as Atletico Madrid beat Celta Vigo 1-0.

Their win, Atletico's ninth straight victory at home this season, moves the club from the capital to within six points of Barca, in second position.

Earlier on Friday, Valencia beat Getafe 4-2 at home to make it two wins out of three for new manager Ernesto Valverde.

Saturday's fixtures:
Betis v Mallorca (1500 GMT), Valladolid v Barcelona (1700 GMT), Osasuna v Granada (1900 GMT), Malaga v Real Madrid (1900 GMT), Athletic Bilbao v Zaragoza (2100 GMT)

- AFP/al



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HC vacates stay on results for 14,000 new PGT posts

CHANDIGARH: While vacating the stay on the announcement of results for around 14,000 new posts of Post Graduate Teachers (PGTs), the Punjab and Haryana high court on Friday held that only those who clear the Haryana Teachers Eligibility Test (HTET) would be eligible for teaching posts in Haryana.

With these orders, candidates having qualified the eligibility test of state, other than Haryana or Central Teachers Eligibility Test (CTET), will not be eligible for these teaching posts in the state.

A division bench headed by chief justice A K Sikri also upheld the condition of Haryana School Teachers Selection Board (HSTSB) that all candidates with four years of teaching experience are eligible for appointments if they qualify HTET before 2015.

The bench passed these orders while dismissing a petition filed by some HTET qualified candidates, who had sought directions to quash the selection criteria which allowed candidates, who have not qualified HTET but are working in any recognized school for the last four years, to become eligible for these posts.

In June this year, Haryana had advertised around 14,000 posts of PGTs in which the government had given relaxation to those candidates from qualifying HTET having teaching experience of four years.

The petitioners had contended that the government was exempting the candidates from HTET just to accommodate the guest teachers working in various schools of the state for last so many years.

Acting on their petition, the HC had restrained HSTSB from declaring the final result of selection in August this year.

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Pictures: Fungi Get Into the Holiday Spirit


Photograph courtesy Stephanie Mounaud, J. Craig Venter Institute

Mounaud combined different fungi to create a Santa hat and spell out a holiday message.

Different fungal grow at different rates, so Mounaud's artwork rarely lasts for long. There's only a short window of time when they actually look like what they're suppose to.

"You do have to keep that in perspective when you're making these creations," she said.

For example, the A. flavus fungi that she used to write this message from Santa grows very quickly. "The next day, after looking at this plate, it didn't say 'Ho Ho Ho.' It said 'blah blah blah,'" Mounaud said.

The message also eventually turned green, which was the color she was initially after. "It was a really nice green, which is what I was hoping for. But yellow will do," she said.

The hat was particularly challenging. The fungus used to create it "was troubling because at different temperatures it grows differently. The pigment in this one forms at room temperature but this type of growth needed higher temperatures," Mounaud said.

Not all fungus will grow nicely together. For example, in the hat, "N. fischeri [the brim and ball] did not want to play nice with the P. marneffei [red part of hat] ... so they remained slightly separated."

Published December 21, 2012

Read More..

Obama Still an 'Optimist' on Cliff Deal


gty barack obama ll 121221 wblog With Washington on Holiday, President Obama Still Optimist on Cliff Deal

Mandel Ngan/AFP/Getty Images


WASHINGTON D.C. – Ten days remain before the mandatory spending cuts and tax increases known as the “fiscal cliff” take effect, but President Obama said he is still a “hopeless optimist” that a federal budget deal can be reached before the year-end deadline that economists agree might plunge the country back into recession.


“Even though Democrats and Republicans are arguing about whether those rates should go up for the wealthiest individuals, all of us – every single one of us -agrees that tax rates shouldn’t go up for the other 98 percent of Americans, which includes 97 percent of small businesses,” he said.


He added that there was “no reason” not to move forward on that aspect, and that it was “within our capacity” to resolve.


The question of whether to raise taxes on incomes over $250,000 remains at an impasse, but is only one element of nuanced legislative wrangling that has left the parties at odds.


For ABC News’ breakdown of the rhetoric versus the reality, click here.


At the White House news conference this evening, the president confirmed he had spoken today to Senate Majority Leader Harry Reid, D-Nev., and House Speaker John Boehner, R-Ohio, although no details of the conversations were disclosed.


The talks came the same day Speaker Boehner admitted “God only knows” the solution to the gridlock, and a day after mounting pressure from within his own Republican Party forced him to pull his alternative proposal from a prospective House vote. That proposal, ”Plan B,” called for extending current tax rates for Americans making up to $1 million a year, a far wealthier threshold than Democrats have advocated.


Boehner acknowledged that even the conservative-leaning “Plan B” did not have the support necessary to pass in the Republican-dominated House, leaving a resolution to the fiscal cliff in doubt.


“In the next few days, I’ve asked leaders of Congress to work towards a package that prevents a tax hike on middle-class Americans, protects unemployment insurance for 2 million Americans, and lays the groundwork for further work on both growth and deficit reduction,” Obama said. ”That’s an achievable goal.  That can get done in 10 days.”


Complicating matters: The halls of Congress are silent tonight. The House of Representatives began its holiday recess Thursday and Senate followed this evening.


Meanwhile, the president has his own vacation to contend with. Tonight, he was embarking for Hawaii and what is typically several weeks of Christmas vacation.


However, during the press conference the president said he would see his congressional colleagues “next week” to continue negotiations, leaving uncertain how long Obama plans to remain in the Aloha State.


The president said he hoped the time off would give leaders “some perspective.”


“Everybody can cool off; everybody can drink some eggnog, have some Christmas cookies, sing some Christmas carols, enjoy the company of loved ones,” he said. “And then I’d ask every member of Congress, while they’re back home, to think about that.  Think about the obligations we have to the people who sent us here.


“This is not simply a contest between parties in terms of who looks good and who doesn’t,” he added later. “There are real-world consequences to what we do here.”


Obama concluded by reiterating that neither side could walk away with “100 percent” of its demands, and that it negotiations couldn’t remain “a contest between parties in terms of who looks good and who doesn’t.”


Boehner’s office reacted quickly to the remarks, continuing recent Republican statements that presidential leadership was at fault for the ongoing gridlock.


“Though the president has failed to offer any solution that passes the test of balance, we remain hopeful he is finally ready to get serious about averting the fiscal cliff,” Boehner said. “The House has already acted to stop all of the looming tax hikes and replace the automatic defense cuts. It is time for the Democratic-run Senate to act, and that is what the speaker told the president tonight.”


The speaker’s office said Boehner “will return to Washington following the holiday, ready to find a solution that can pass both houses of Congress.”


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Administration says furloughs are possible, but not immediately



They don’t necessarily think it is going to happen.


But Obama administration officials want federal employees to be ready if it does happen.

“It” is sequestration, the process of across-the-board budget cuts that are scheduled to begin taking effect in January unless the White House and Congress come to an agreement to prevent “it.”

To get folks ready, federal agencies sent messages to their employees Thursday to explain what sequestration is and is not and how it would, and would not, affect operations, albeit in general terms.

“Under sequestration, we would still have funds available after January 2, but our overall funding for the remainder of the year would be reduced,” said a message from “Charlie B,” also known as NASA Administrator Charles F. Bolden Jr.

Nearly identical language, supplied by the Office of Management and Budget, was in messages sent by agencies.

Explaining that sequestration — which was put into place by the Budget Control Act of 2011 — is different from a government shutdown in which agency appropriations have lapsed, the message from Bolden and others added: “For these reasons, I do not expect our day-to-day operations to change dramatically on or immediately after January 2, should sequestration occur. This means that we will not be executing any immediate personnel actions, such as furloughs, on that date. Should we have to operate under reduced funding levels for an extended period of time, we may have to consider furloughs or other actions in the future.”

William R. Dougan, president of the National Federation of Federal Employees, who was among the labor leaders on a 6 p.m. Wednesday conference call with Office of Personnel Management Director John Berry and Danny Werfel, controller of the Office of Management and Budget, said the notice to employees would help prevent them from being alarmed during this period of great uncertainty in government.

“Let me assure you,” the messages to employees said, “that we will carefully examine other options to reduce costs within the agency before taking such [personnel] action, taking into consideration our obligation to execute our core mission.”

Layoffs, or reductions in force, were not mentioned in the messages or predicted by administration officials during the call, but the possibility of “other actions in the future” clearly makes labor leaders wary.

“Sequestration would very likely lead to furloughs and RIFs,” said J. David Cox, president of the American Federation of Government Employees, “so will the spending caps which are also part of the Budget Control Act — the law whose terrible provisions will undermine federal employees and agencies for an entire decade.”

Despite that prediction, Rep. James Moran (D-Va.), whose northern Virginia district is home to about 165,000 current and former federal employees, said he worries they still don’t understand the real possibility of furloughs and layoffs.

“They really are taking this with kind of as casual an attitude as Wall Street is taking it,” he said. “But by the time we get to next week, between Christmas and New Year’s, all of a sudden they’re going to realize how serious this is. This OMB notice may alert them that this is real. I don’t think most of them accept that this is real yet, even though they’ve been reading about it. They think Congress has been doing its normal thing. I’m very much worried, it’s going to be chaos if we go over the cliff, and I think there’s at least a 50-50 chance that we’ll go over.”

Cox said he’s “glad the administration seems to be taking a ‘hope for the best, but prepare for the worst’ approach, because as awful as sequestration would be, some of the items in these ‘grand deals’ are outrageously bad for federal employees. We’re doing our own preparation to make sure that if sequestration does occur, agencies spread the pain to their larger and more costly contractor workforce and don’t put it all on us.”

The International Federation of Professional & Technical Engineers, which reported on the conference call in a “sequestration update” e-mail to its members Thursday morning, said this:

“First and foremost, it is becoming more and more likely that there will be a deal that includes significant revenue increases so that there will be less of a need for cuts. Even if it doesn’t happen by December 31st, it will likely happen by early January at the absolute latest.

“Second, regardless of the final deal, there will be large cuts to most federal agencies so there will be pain although much less than if the Budget Control Act (sequestration) were allowed to kick in.

“Third, sequestration or any deal to avoid it is not a shutdown. All federal agencies will be open on January 2nd, and all federal employees should report to work as scheduled. Any cuts will be gradually implemented and so there should not be any real disruptions in January (i.e., no furloughs).”

That’s about as optimistic an outlook of the current situation as you’ll find. But even it clearly indicates there will be pain.

The question is how much pain, how soon.

Ed O’Keefe contributed to this report. Previous columns by Joe Davidson are available at wapo.st/JoeDavidson.

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Gujarat election result: Gujarat Parivartan Party shrinks BJP win

RAJKOT: Former chief minister Keshubhai Patel's call for parivartan (change) may have met with indifference. But his Gujarat Parivartan Party (GPP) denied BJP a sweeter victory in the saffron stronghold Saurashtra. In absence of GPP, BJP was set to break the astronomical figure of 130 seats.

Results show that GPP presence has cost BJP at least 10 seats in Saurashtra namely Talala, Lathi, Palitana, Limdi , Wankaner, Rajkot(east), Jasdan, Jetpur, Dhoraji and Manavadar.

While Keshubhai himself trounced sitting MLA Kanu Bhalala in Visavadar, GPP's Nalin Kotadiya too defeated Dhari legislator Mansukh Bhuva to grab the seat. A former BJP man, Kotadiya had shifted loyalty to Keshubhai two months ago.

GPP played a spoilsport in Wankaner too where BJP had a bright chance of winning the seat back from Congress. Had GPP's Parsottam Bavalava not pocketed around 20,000 votes, BJP's Jitu Somani was sure to win considering that the sitting Congress MLA Javed Pirzada won by just 5,000 votes.

In Rajkot (east), BJP fielded Kashyap Shukla, son of one of BJP founders in state Chiman Shukla. But GPP's Pravin Ambaliya bagged around 15,000-20 ,000 votes, paving way for Congress to win this traditional BJP seat for the first time.

BJP's sitting minister of state for forests Kiritsinh Rana too has only GPP's candidate Pravinsinh Solanki to blame for his defeat. Solanki hails from Darbar community , the same as Rana's . He bagged 2,560 votes that led to Congress's Soma Ganda Patel scraping through by just 1,561.

GPP's Nathu Kamaliya also denied victory to BJP's Govind Parmar in Talala seat of Junagadh district by grabbing nearly 10,599 votes. There are around 20,000 Leuva Patels too in this constituency . The defeat of state BJP president RC Faldu's loss in Jamnagar (rural) seat is also being attributed to GPP's Pranjivan Kundaria.

Meanwhile, the ignominious defeat of Keshubhai's party also brought to fore that fact that regional parties or third fronts cannot become a force to reckon with. In the past, regional parties like Swatantra Party, Maha Saurashtra P arty, Maha Gujarat Janta Parishad, Rashtriya Congress, Kisan Majdoor Lok Paksha and Maha Gujarat Janta Party have tried to test their electoral mettle. But all remained a one-election wonder. "People in Gujarat do not accept regional parties. The main reason is that such parties don't have organizational support and rank and file to build the party," said political analyst.

Shankersinh Vaghela's infamous rebellion against BJP to form Rashtriya Janta Party (RJP) did not help the former chief minister for long. In the ensuing elections, voters ou rightly rejected RJP got just four seats.

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Hollies Get Prickly for a Reason



With shiny evergreen leaves and bright red berries, holly trees are a naturally festive decoration seen throughout the Christmas season.


They're famously sharp. But not all holly leaves are prickly, even on the same tree. And scientists now think they know how the plants are able to make sharper leaves, seemingly at will. (Watch a video about how Christmas trees are made.)


A new study published in the Botanical Journal of the Linnean Society suggests leaf variations on a single tree are the combined result of animals browsing on them and the trees' swift molecular response to that sort of environmental pressure.


Carlos Herrera of the National Research Council of Spain led the study in southeastern Spain. He and his team investigated the European holly tree, Ilex aquifolium. Hollies, like other plants, can make different types of leaves at the same time. This is called heterophylly. Out of the 40 holly trees they studied, 39 trees displayed different kinds of leaves, both prickly and smooth.



Five holly leaves from the same tree.

Five holly leaves from the same tree.


Photographs by Emmanuel Lattes, Alamy




Some trees looked like they had been browsed upon by wild goats and deer. On those trees, the lower 8 feet (2.5 meters) had more prickly leaves, while higher up the leaves tended to be smooth. Scientists wanted to figure out how the holly trees could make the change in leaf shape so quickly.


All of the leaves on a tree are genetic twins and share exactly the same DNA sequence. By looking in the DNA for traces of a chemical process called methylation, which modifies DNA but doesn't alter the organism's genetic sequence, the team could determine whether leaf variation was a response to environmental or genetic changes. They found a relationship between recent browsing by animals, the growth of prickly leaves, and methylation.


"In holly, what we found is that the DNA of prickly leaves was significantly less methylated than prickless leaves, and from this we inferred that methylation changes are ultimately responsible for leaf shape changes," Herrera said. "The novelty of our study is that we show that these well-known changes in leaf type are associated with differences in DNA methylation patterns, that is, epigenetic changes that do not depend on variation in the sequence of DNA."


"Heterophylly is an obvious feature of a well-known species, and this has been ascribed to browsing. However, until now, no one has been able to come up with a mechanism for how this occurs," said Mike Fay, chief editor of the Botanical Journal of the Linnean Society and head of genetics at the Kew Royal Botanic Gardens. "With this new study, we are now one major step forward towards understanding how."


Epigenetic changes take place independently of variation in the genetic DNA sequence. (Read more about epigenetics in National Geographic magazine's "A Thing or Two About Twins.")


"This has clear and important implications for plant conservation," Herrera said. In natural populations that have their genetic variation depleted by habitat loss, the ability to respond quickly, without waiting for slower DNA changes, could help organisms survive accelerated environmental change. The plants' adaptability, he says, is an "optimistic note" amidst so many conservation concerns. (Related: "Wild Holly, Mistletoe, Spread With Warmer Winters.")


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